GAS-CHROMATOGRAPHIC MASS-SPECTROMETRIC CHARACTERIZATION OF SOME NOVEL HYDROXYEICOSATETRAENOIC ACIDS FORMED ON INCUBATION OF ARACHIDONIC-ACID WITH MICROSOMES FROM INDUCED RAT LIVERS

被引：21

作者：

CLARE, RA ^{[1
]}

HUANG, S ^{[1
]}

DOIG, MV ^{[1
]}

GIBSON, GG ^{[1
]}

机构：

[1] UNIV SURREY,DEPT BIOCHEM,GUILDFORD GU2 5XH,SURREY,ENGLAND

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 562卷 / 1-2期

基金：

英国惠康基金;

关键词：

D O I：

10.1016/0378-4347(91)80581-V

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The biotransformation of arachidonic acid by rat liver microsomes from both control animals and animals pretreated with known inducers of cytochrome P-450 isoenzymes has been studied using a combination of reversed- and normal-phase high-performance liquid chromatography and combined gas chromatography-electron-impact mass spectrometry. The metabolite profiles observed were found to be dependent upon the inducing agent. Five metabolites were identified, namely 16-, 17-, 18-, 19- and 20-hydroxylated arachidonic acids. Of these the 16- and 17-isomers have not been reported as products of arachidonic acid metabolism by any biological system and the 18-isomer has not been reported as a product of liver metabolism.

引用

页码：237 / 247

页数：11

共 12 条

[1] IMMUNOCHEMICAL STUDY ON THE CONTRIBUTION OF HYPOLIPIDEMIC-INDUCED CYTOCHROME-P-452 TO THE METABOLISM OF LAURIC ACID AND ARACHIDONIC-ACID
BAINS, SK
GARDINER, SM
MANNWEILER, K
GILLETT, D
GIBSON, GG
[J]. BIOCHEMICAL PHARMACOLOGY, 1985, 34 (18) : 3221 - 3229
[2] INFLUENCE OF A FIBRIC ACID TYPE OF HYPOLIPIDEMIC AGENT ON THE OXIDATIVE-METABOLISM OF ARACHIDONIC-ACID BY LIVER MICROSOMAL CYTOCHROME-P-450
CAPDEVILA, J
KIM, YR
WIXTROM, CM
FALCK, JR
MANNA, S
ESTABROOK, RW
[J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1985, 243 (01) : 8 - 19
[3] CYTOCHROME P-450-DEPENDENT OXYGENATION OF ARACHIDONIC-ACID TO HYDROXYICOSATETRAENOIC ACIDS
CAPDEVILA, J
MARNETT, LJ
CHACOS, N
PROUGH, RA
ESTABROOK, RW
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (03): : 767 - 770
[4] CAPDEVILA J, 1982, P NATL ACAD SCI USA, V78, P5363
[5] FITZPATRICK FA, 1989, PHARMACOL REV, V40, P229
[6] CYTOCHROME-P-450 ISOENZYMES INVOLVED IN FATTY-ACID METABOLISM
GIBSON, GG
BAINS, SK
[J]. BIOCHEMICAL SOCIETY TRANSACTIONS, 1985, 13 (05) : 850 - 852
[7] OLIW EH, 1981, J BIOL CHEM, V256, P9924
[8] OLIW EH, 1989, J BIOL CHEM, V264, P17845
[9] REGULATION OF ARACHIDONIC-ACID METABOLISM BY CYTOCHROME-P-450 IN RABBIT KIDNEY
SCHWARTZMAN, ML
ABRAHAM, NG
CARROLL, MA
LEVERE, RD
MCGIFF, JC
[J]. BIOCHEMICAL JOURNAL, 1986, 238 (01) : 283 - 290
[10] MICROSOMAL CYTOCHROME-P-452 INDUCTION AND PEROXISOME PROLIFERATION BY HYPOLIPEMIC AGENTS IN RAT-LIVER - A MECHANISTIC INTER-RELATIONSHIP
SHARMA, R
LAKE, BG
FOSTER, J
GIBSON, GG
[J]. BIOCHEMICAL PHARMACOLOGY, 1988, 37 (07) : 1193 - 1201

← 1 2 →