DETECTION OF VARICELLA-ZOSTER VIRUS (VZV) DNA IN CLINICAL-SAMPLES FROM PATIENTS WITH VZV BY THE POLYMERASE CHAIN-REACTION

被引：58

作者：

KIDO, S

OZAKI, T

ASADA, H

HIGASHI, K

KONDO, K

HAYAKAWA, Y

MORISHIMA, T

TAKAHASHI, M

YAMANISHI, K

机构：

[1] OSAKA UNIV,MICROBIAL DIS RES INST,DEPT VIROL,SUITA,OSAKA 565,JAPAN

[2] NAGOYA UNIV,SCH MED,DEPT PEDIAT,NAGOYA,AICHI 466,JAPAN

[3] SHOWA HOSP,KONAN,AICHI,JAPAN

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1991年 / 29卷 / 01期

关键词：

D O I：

10.1128/JCM.29.1.76-79.1991

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A polymerase chain reaction system for the detection of varicella-zoster virus was established. Of 25 nucleotides, 4 oligonucleotide pairs (regions of thymidine kinase, thymidylate synthetase, glycoprotein I, and immediate early gene) were synthesized. The first three oligonucleotide pairs could be used as primers on the basis of specific DNA amplification. Varicella-zoster virus DNA was amplified by this polymerase chain reaction system in 20 of 20 vesicle samples, 5 of 6 crusts, and 12 of 13 throat swabs collected from patients with clinical varicella.

引用

页码：76 / 79

页数：4

共 9 条

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