TRANSFER OF THE GLU-D1 GENE FROM CHROMOSOME-1D OF BREADWHEAT TO CHROMOSOME-1R IN HEXAPLOID TRITICALE

The use of hexaploid triticale as a crop for human consumption has been limited by its inferior bread-making quality. To ameliorate this problem, a segment of chromosome 1D of breadwheat with the Glu-D1d allele encoding for high molecular weight glutenin subunits 5 + 10 was translocated to chromosome 1R of the hexaploid triticale 'Rhino' through a combination of a centric break-fusion translocation followed by 5D(5B)-induced homoeologous pairing. The resulting recombinant chromosome 1R has a small interstitial segment of 1D with the Glu-D1d allele. The maximum physical length of the translocated segment is estimated at about 16.5 % of 1DL. Frequency of translocations involving the long arms of homoeologous group-1 chromosomes in the analyzed progeny suggested that homoeologous recombination in triticale was substantially higher than that previously reported in hexaploid wheat.