PURIFICATION OF A STREPTOCOCCAL BACTERIOCIN (VIRIDIN-B) AND ITS SEPARATION FROM ALPHA-HEMOLYSIN

被引：5

作者：

APELGREN, LD ^{[1
]}

DAJANI, AS ^{[1
]}

机构：

[1] WAYNE STATE UNIV, SCH MED, DEPT PEDIAT, DETROIT, MI 48201 USA

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 1979年 / 15卷 / 03期

关键词：

D O I：

10.1128/AAC.15.3.436

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Viridin B, a bacteriocin produced by Streptococcus mitis, copurified with the alpha-hemolysin after ammonium sulfate precipitation and gel filtration on Sephadex G-200. The bacteriocin and hemolysin were separated in some instances by ion-exchange chromatography on diethylaminoethyl-Sephadex A-50, but the two substances were shown to be distinct after polyacrylamide gel electrophoresis. Attempts at recovery of nonhemolytic or nonbacteriocinogenic mutants were unsuccessful after exposure to mutagenic agents. The molecular weight of viridin B was determined to be approximately 87,000.

引用

页码：436 / 439

页数：4

共 11 条

[1] BACTERIOCIN (HEMOLYSIN) OF STREPTOCOCCUS ZYMOGENES [J].

BASINGER, SF ;

JACKSON, RW .

JOURNAL OF BACTERIOLOGY, 1968, 96 (06) :1895-&

[2] PROBABLE IDENTITY OF A GROUP D HEMOLYSIN WITH A BACTERIOCINE [J].

BROCK, TD ;

DAVIE, JM .

JOURNAL OF BACTERIOLOGY, 1963, 86 (04) :708-&

[3]

DAJANI AS, 1977, P SOC EXP BIOL MED, V155, P456

[4] ULTRASTRUCTURAL AND BIOCHEMICAL-ALTERATIONS EFFECTED BY VIRIDIN-B, A BACTERIOCIN OF ALPHA-HEMOLYTIC STREPTOCOCCI [J].

DAJANI, AS ;

LAW, DJ ;

BOLLINGER, RO ;

ECKLUND, PS .

INFECTION AND IMMUNITY, 1976, 14 (03) :776-782

[5] VIRIDINS, BACTERIOCINS OF ALPHA-HEMOLYTIC STREPTOCOCCI - ISOLATION, CHARACTERIZATION, AND PARTIAL-PURIFICATION [J].

DAJANI, AS ;

TOM, MC ;

LAW, DJ .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1976, 9 (01) :81-88

[6] INTERACTIONS BETWEEN NEISSERIA-SICCA AND VIRIDIN-B, A BACTERIOCIN PRODUCED BY STREPTOCOCCUS-MITIS [J].

LAW, DJ ;

DAJANI, AS .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1978, 13 (03) :473-478

[7]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

[8] A NEW CHEMICAL MUTAGEN FOR BACTERIA, 1-METHYL-3-NITRO-1-NITROSOGUANIDINE [J].

MANDELL, JD ;

GREENBERG, J .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1960, 3 (06) :575-577

[9] BACTERIOCINS OF GRAM-POSITIVE BACTERIA [J].

TAGG, JR ;

DAJANI, AS ;

WANNAMAKER, LW .

BACTERIOLOGICAL REVIEWS, 1976, 40 (03) :722-756

[10]

WEBER K, 1969, J BIOL CHEM, V244, P4406

← 1 2 →