INTERACTIONS OF PROTEINS WITH UNIFORM COLLOIDAL HEMATITE AND CHROMIUM HYDROXIDE PARTICLES .2. STABILITY AND MOBILITY

被引:5
作者
JOHNSON, JE [1 ]
MATIJEVIC, E [1 ]
机构
[1] CLARKSON UNIV,DEPT CHEM,POTSDAM,NY 13699
关键词
ELECTROKINETICS OF PROTEINS; FLOCCULATION BY PROTEINS; PROTEIN COATINGS; PROTEIN/METAL OXIDES;
D O I
10.1007/BF00655851
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Stability of hematite and chromium hydroxide particles covered by ovalbumin, gamma-globulin, and lysozyme, respectively, and mobilities of the same coated particles in aqueous media were investigated as a function of the pH, ionic strength [NaNO3, Mg(NO3)2], and the amount of added proteins. It was found that ovalbumin causes electrosteric stabilization of dispersions at pH values other than 5, while flocculation occurred at pH 5 (which was the i.e.p. of the coated particles). Mobility curves of ovalbumin covered particles resembled those of the pure protein. Dispersions with gamma-globulin flocculated at and around the i.e.p. of the macromolecule (pH almost-equal-to 7). The mobility curves of gamma-globulin-coated sols were intermediate between those of the protein and of the cores, and were dependent upon the amount of adsorbed gamma-globulin. Heterocoagulation was observed for both hematite and chromium hydroxide dispersions with lysozyme. Mobilities of lysozyme covered particles were between those of the cores and of the protein, but were not dependent upon the quantity of adsorbed polymer.
引用
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页码:364 / 369
页数:6
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