2-DIMENSIONAL PROTEIN SEPARATION BY MICROCOLUMN SIZE-EXCLUSION CHROMATOGRAPHY CAPILLARY ZONE ELECTROPHORESIS

被引:64
作者
LEMMO, AV [1 ]
JORGENSON, JW [1 ]
机构
[1] UNIV N CAROLINA,DEPT CHEM,CHAPEL HILL,NC 27599
来源
JOURNAL OF CHROMATOGRAPHY | 1993年 / 633卷 / 1-2期
基金
美国国家科学基金会;
关键词
D O I
10.1016/0021-9673(93)83156-M
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A two-dimensional separation system for the study of proteins consisting of size-exclusion chromatography (SEC) and capillary zone electrophoresis (CZE) is presented. The SEC separation was carried out in a 1 m x 250 mum I.D. microcolumn. The effluent from this SEC microcolumn filled a sample loop on a computer controlled 6-port valve. The contents of this loop were swept past the grounded end of the CZE capillary for electromigration injection. Detection was by on-line UV detection at 214 nm. The system was initially tested with protein test mixtures. then was used to analyze human, horse and bovine sera. Data are presented as three-dimensional chromatoelectropherograms and grayscale images.
引用
收藏
页码:213 / 220
页数:8
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