2-DIMENSIONAL PROTEIN SEPARATION BY MICROCOLUMN SIZE-EXCLUSION CHROMATOGRAPHY CAPILLARY ZONE ELECTROPHORESIS

被引：64

作者：

LEMMO, AV ^{[1
]}

JORGENSON, JW ^{[1
]}

机构：

[1] UNIV N CAROLINA,DEPT CHEM,CHAPEL HILL,NC 27599

来源：

JOURNAL OF CHROMATOGRAPHY | 1993年 / 633卷 / 1-2期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/0021-9673(93)83156-M

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A two-dimensional separation system for the study of proteins consisting of size-exclusion chromatography (SEC) and capillary zone electrophoresis (CZE) is presented. The SEC separation was carried out in a 1 m x 250 mum I.D. microcolumn. The effluent from this SEC microcolumn filled a sample loop on a computer controlled 6-port valve. The contents of this loop were swept past the grounded end of the CZE capillary for electromigration injection. Detection was by on-line UV detection at 214 nm. The system was initially tested with protein test mixtures. then was used to analyze human, horse and bovine sera. Data are presented as three-dimensional chromatoelectropherograms and grayscale images.

引用

页码：213 / 220

页数：8

共 13 条

[1] CAPILLARY ELECTROPHORESIS OF PROTEINS IN BUFFERS CONTAINING HIGH-CONCENTRATIONS OF ZWITTERIONIC SALTS [J].