REGULATION OF UNDULIN SYNTHESIS AND GENE-EXPRESSION IN HUMAN FAT-STORING CELLS BY ACETALDEHYDE AND TRANSFORMING GROWTH-FACTOR-BETA-1 - COMPARISON WITH FIBRONECTIN

被引：27

作者：

CASINI, A ^{[1
]}

CENI, E ^{[1
]}

SALZANO, R ^{[1
]}

SCHUPPAN, D ^{[1
]}

MILANI, S ^{[1
]}

PELLEGRINI, G ^{[1
]}

SURRENTI, C ^{[1
]}

机构：

[1] FREE UNIV BERLIN,KLINIKUM STEGLITZ,DEPT GASTROENTEROL,BERLIN,GERMANY

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 199卷 / 02期

关键词：

D O I：

10.1006/bbrc.1994.1331

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Undulin and fibronectin (FN) are large extracellular matrix (ECM) glycoproteins possibly involved in cell-matrix interactions. In this study we analyzed the effect of acetaldehyde and transforming growth factor-beta 1 (TGF-beta 1) on undulin and FN synthesis in cultured fat-storing cells (FSC) isolated from wedge sections of normal human livers. Cultured human FSC expressed two mRNA transcripts (6.5 and 8.5 kb) specific for undulin. Acetaldehyde inhibited both undulin mRNA and protein expression, whereas it had an opposite (stimulatory) effect on FN synthesis. TGF-beta 1 induced a dose-dependent increase of both undulin and FN synthesis in FSC cultures. Furthermore, TGF-beta 1 antagonized the inhibitory effect of acetaldehyde on undulin production and potentiated the stimulatory effect of acetaldehyde on FN synthesis. Since undulin is involved in the supramolecular organization of fibrillar collagens and in their enzymatic degradation, its acetaldehyde-induced inhibition may contribute to ECM rearrangement in the early stages of alcoholic liver fibrosis. (C) 1994 Academic Press, Inc.

引用

页码：1019 / 1026

页数：8

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