CLUSTERIN EXPRESSION BY ASTROCYTES IS INFLUENCED BY TRANSFORMING GROWTH-FACTOR-BETA-1 AND HETEROTYPIC CELL-INTERACTIONS

被引：74

作者：

MORGAN, TE ^{[1
]}

LAPING, NJ ^{[1
]}

ROZOVSKY, I ^{[1
]}

ODA, T ^{[1
]}

HOGAN, TH ^{[1
]}

FINCH, CE ^{[1
]}

PASINETTI, GM ^{[1
]}

机构：

[1] UNIV SO CALIF,DEPT BIOL SCI,LOS ANGELES,CA 90089

来源：

JOURNAL OF NEUROIMMUNOLOGY | 1995年 / 58卷 / 01期

关键词：

TRANSFORMING GROWTH FACTOR BETA-1; CLUSTERIN; MESSENGER-RNA TRANSCRIPTION; ASTROCYTES; MICROGLIA;

D O I：

10.1016/0165-5728(94)00194-S

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

This study characterizes the effect of transforming growth factor (TGF) beta 1 on clusterin expression in rat brain cells. 24 h after an acute unilateral intracerebroventricular infusion of TGF-beta 1, clusterin mRNA prevalence was increased in astrocytes that contained immunoreactive (IR) glial fibrillary acidic protein (GFAP). TGF-beta 1 selectively induced clusterin mRNA in astrocytes, as no clusterin mRNA was detected in neurons, oligodendrocytes, or microglia. TGF-beta 1 induced a bilateral increase in clusterin mRNA per astrocyte. Astrocyte hypertrophy (GFAP-IR area) was only increased on the ipsilateral side. In pure astrocyte cultures, TGF-beta 1 (200 pM) decreased clusterin mRNA levels and the rate of clusterin RNA transcription. However, in cultures of astrocytes that contained microglia and oligodendrocytes (mixed glia cultures), TGF-beta 1 caused a dose-dependent increase in astrocytic clusterin mRNA levels. The astrocytes that responded to TGF-beta 1 included two GFAP-IR subtypes, type 1 and 2. TGF-beta 1 increased clusterin protein in the conditioned medium from cultured glia, in either monotypic or mixed glial cultures. Thus, TGF-beta 1 and heterotypic cell interactions influence clusterin expression by astrocytes and may be important to the role of clusterin in multiple sclerosis, AIDS, and Alzheimer's disease.

引用

页码：101 / 110

页数：10

共 49 条

[1] TYPE-I RECEPTORS SPECIFY GROWTH-INHIBITORY AND TRANSCRIPTIONAL RESPONSES TO TRANSFORMING GROWTH-FACTOR-BETA AND ACTIVIN [J].