RAPID PROTEIN-ANALYSIS BY CAPILLARY ELECTROPHORESIS

Rapid protein analysis by free-zone capillary electrophoresis in an untreated fused-silica column is presented. Separation of model proteins by the proposed method shows that the retention times of these proteins correlate well with each of their isoelectric points. By using a high voltage gradient, most proteins could be separated in 200 s with excellent reproducibility. Similarly, human serum proteins could be resolved and determined in less than 100 s.