GENE CENTROMERE LINKAGE MAPPING BY PCR ANALYSIS OF INDIVIDUAL OOCYTES

被引：21

作者：

CUI, XF

GERWIN, J

NAVIDI, W

LI, HH

KUEHN, M

ARNHEIM, N

机构：

[1] UNIV SO CALIF, DEPT MATH, LOS ANGELES, CA 90089 USA

[2] UNIV ILLINOIS, COLL MED, DEPT GENET, CHICAGO, IL 60612 USA

来源：

GENOMICS | 1992年 / 13卷 / 03期

关键词：

D O I：

10.1016/0888-7543(92)90146-J

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We describe a general method of determining the recombination fraction between a polymorphic locus and the centromere in any species where single oocytes can be obtained. After removal of the first polar body, each oocyte is analyzed by PCR. The frequency of oocytes heterozygous at the polymorphic locus is used to estimate the recombination fraction. We estimate a recombination fraction of 0.15 between the mouse major histocompatibility complex (H-2) and the centromere of chromosome 17. © 1992.

引用

页码：713 / 717

页数：5

共 43 条

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