CHLORAMPHENICOL AND NITROFURAN RESIDUE ANALYSIS BY HPLC AND PHOTODIODE ARRAY DETECTION IN MEAT AND FISH

This paper describes a chromatographic method for the analysis of residues of chloramphenicol and nitrofurans in meat and fish. Chloramphenicol, furazolidone, furaltadone and nitrofurazone were extracted from the tissues with ethyl acetate. The extract was purified with petroleum ether and n-pentane and then analyzed with a liquid chromatograph and a photodiode array detector using a Chromspher C-8 column. Chloramphenicol and nitrofurans were detected at 280 nm and 360 nm respectively. Blank samples fortified with chloramphenicol and nitrofurans at levels of 5, 10 and 20-mu-g/kg gave recoveries in the range of 69 to 88%. A quantitation limit of 1-mu-g/kg for nitrofurazone and furazolidone and of 2-mu-g/kg for chloramphenicol and furaltadone in meat and fish has been reached.