COLONY IMMUNOBLOT ASSAY OF BOTULINAL TOXIN

被引：23

作者：

GOODNOUGH, MC

HAMMER, B

SUGIYAMA, H

JOHNSON, EA

机构：

[1] UNIV WISCONSIN,DEPT FOOD MICROBIOL & TOXICOL,1925 WILLOW DR,MADISON,WI 53706

[2] UNIV WISCONSIN,DEPT BACTERIOL,MADISON,WI 53706

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1993年 / 59卷 / 07期

关键词：

D O I：

10.1128/AEM.59.7.2339-2342.1993

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Botulinal neurotoxin in and around colonies of Clostridium botulinum types A, B, and E and of toxigenic Clostridium butyricum was detected by an enzyme-linked immunoassay procedure whereby the toxin was transferred from the agar medium to a nitrocellulose support and the immobilized toxin was probed with type-specific antibodies. The method identified the toxin types of the colonies grown from a mixed inoculum of C. botulinum serotypes. The specificity of the antitoxins for type A and B toxins was improved by adsorption of the antitoxins with the antigens of heterologous type cultures.

引用

页码：2339 / 2342

页数：4

共 12 条

[1] STATISTICAL ANALYSIS OF A RAPID IN VIVO METHOD FOR TITRATION OF TOXIN OF CLOSTRIDIUM BOTULINUM [J].