COLONY IMMUNOBLOT ASSAY OF BOTULINAL TOXIN

被引:23
作者
GOODNOUGH, MC
HAMMER, B
SUGIYAMA, H
JOHNSON, EA
机构
[1] UNIV WISCONSIN,DEPT FOOD MICROBIOL & TOXICOL,1925 WILLOW DR,MADISON,WI 53706
[2] UNIV WISCONSIN,DEPT BACTERIOL,MADISON,WI 53706
关键词
D O I
10.1128/AEM.59.7.2339-2342.1993
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Botulinal neurotoxin in and around colonies of Clostridium botulinum types A, B, and E and of toxigenic Clostridium butyricum was detected by an enzyme-linked immunoassay procedure whereby the toxin was transferred from the agar medium to a nitrocellulose support and the immobilized toxin was probed with type-specific antibodies. The method identified the toxin types of the colonies grown from a mixed inoculum of C. botulinum serotypes. The specificity of the antitoxins for type A and B toxins was improved by adsorption of the antitoxins with the antigens of heterologous type cultures.
引用
收藏
页码:2339 / 2342
页数:4
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