ADENOSINE-DEAMINASE-DEFICIENT MICE DIE PERINATALLY AND EXHIBIT LIVER-CELL DEGENERATION, ATELECTASIS AND SMALL-INTESTINAL CELL-DEATH

We report the generation and characterization of mice lacking adenosine deaminase (ADA). in humans, absence of ADA causes severe combined immunodeficiency. In contrast, ADA-deficient mice die perinatally with marked liver-cell degeneration, but lack abnormalities in the thymus. The ADA substrates, adenosine and deoxyadenosine, are increased in ADA-deficient mice. Adenine deoxyribonucleotides are only modestly elevated, whereas S-adenosylhomocysteine hydrolase activity is reduced more than 85%. Consequently, the ratio of S-adenosylhomocysteine (AdoMet) to S-adenosyl homocysteine (AdoHcy) is reduced threefold in liver. We conclude that ADA plays a more critical role in murine than human fetal development. me murine liver pathology may be due to AdoHcy-mediated inhibition of AdoMet-dependent transmethylation reactions.