NMDA-DEPENDENT NGF MESSENGER-RNA EXPRESSION BY HUMAN ASTROCYTOMA-CELLS IS MEDIATED BY NITRIC-OXIDE

被引：6

作者：

PERSICHINI, T

COLASANTI, M

MOLLACE, V

NISTICO, G

RAMACCI, MT

LAURO, GM

机构：

[1] UNIV ROME 3,DEPT BIOL,I-00154 ROME,ITALY

[2] IRS SIGMA TAU,POMEZIA,ITALY

[3] UNIV ROMA TOR VERGATA,DEPT BIOL,I-00173 ROME,ITALY

来源：

NEUROREPORT | 1994年 / 5卷 / 18期

关键词：

NITRIC OXIDE; NMDA; NERVE GROWTH FACTOR; ASTROCYTOMA; CYCLIC GMP; RT-PCR;

D O I：

10.1097/00001756-199412000-00018

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

THE aim of this study was to investigate the role of NO-cGMP pathway in NMDA-induced NGF mRNA expression by T67 astrocytoma cells. Levels of nitrite, a breakdown product of NO, in supernatants of NMDA-treated astrocytoma cells were significantly higher compared with control cells, this effect being reversed by the specific NO synthase inhibitor L-NAME. Furthermore, NGF mRNA expression was induced by NMDA treatment, this effect being inhibited by pretreating cells with L-NAME. Moreover, methylene blue, an inhibitor of NO biological activity at guanylate cyclase level, inhibited NMDA-induced NGF mRNA expression and this effect was reversed by dbt(2)-cGMP. These findings suggest that NO-cGMP pathway mediates the synthesis of NGF mRNA.

引用

页码：2477 / 2480

页数：4

共 24 条

[1] REGULATION OF NERVE GROWTH-FACTOR AND NERVE GROWTH-FACTOR RECEPTOR PRODUCTION BY NMDA IN C6 GLIOMA-CELLS [J].