COMPARATIVE STUDIES ON 6 BLOOD-SERUM GLYCOSIDASES FROM SEVERAL MAMMALIAN-SPECIES

1. 1. Peripheral blood serum α-d-galactosidase, β-d-galactosidase, β-d-glucosidase, α-d-mannosidase, β-d-xylosidase and β-d-glucuronidase have been studied with a comparative point of view from several mammalian species: Bos taurus L. (bull), Capra hircus L. (goat), Sus scropha var. domestica L. (pig) and man. 2. 2. Fluorimetric and spectrophotometric procedures were used for determination of enzyme activities and pH optima. 3. 3. Glycosidase activity was generally higher with fluorescent substrates than with chromogenic substrates. 4. 4. α-d-mannonidase was the most active with both fluorescent and chromogenic substrates. 5. 5. All the studied enzymes had the same pH optimum (4.0) when the chromogenic substrates were used. 6. 6. pH optima of these glycosidases ranged from 3.0 to 5.5 when the fluorescent substrates were used. © 1979.