FRACTIONATION AND ANTIGENIC CHARACTERIZATION OF ORGANELLES OF EIMERIA-TENELLA SPOROZOITES

被引：41

作者：

KAWAZOE, U ^{[1
]}

TOMLEY, FM ^{[1
]}

FRAZIER, JA ^{[1
]}

机构：

[1] INST ANIM HLTH, HOUGHTON LAB, HUNTINGDON PE17 2DA, CAMBS, ENGLAND

来源：

PARASITOLOGY | 1992年 / 104卷

关键词：

EIMERIA-TENELLA; SPOROZOITES; ORGANELLES; ANTIGENS;

D O I：

10.1017/S003118200006073X

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Sporozoites of Eimeria tenella were disrupted by sonication and subcellular fractions were separated by sucrose gradient ultracentrifugation. Fractions from gradients were characterized by electron microscopical appearance and their polypeptide and antigenic profiles determined by PAGE and Western blotting with antisera to sporozoites and 1st- and 2nd-generation merozoites. Fractions containing micronemes, rhoptries or membranes showed markedly different polypeptide content and antigenic reactivity. Microneme epitopes were strongly conserved between sporozoites and 2nd-generation merozoites whereas the majority of rhoptry epitopes and many membrane epitopes were sporozoite specific. The only polypeptide of sporozoites which was strongly recognized by antisera raised to 1st generation merozoites was a microneme antigen of molecular weight approximately 100 kDa.

引用

页码：1 / 9

页数：9

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