HOMOLOGOUS RECOMBINATION PROMOTED BY REVERSE-TRANSCRIPTASE DURING COPYING OF 2 DISTINCT RNA TEMPLATES

被引：36

作者：

NEGRONI, M ^{[1
]}

RICCHETTI, M ^{[1
]}

NOUVEL, P ^{[1
]}

BUC, H ^{[1
]}

机构：

[1] INST PASTEUR,UNITE GENET MAMMIFERES,F-75724 PARIS 15,FRANCE

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1995年 / 92卷 / 15期

关键词：

D O I：

10.1073/pnas.92.15.6971

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Retroviruses are known to mutate at high rates. An important source of genetic variability is recombination taking place during reverse transcription of internal regions of the two genomic RNAs. We have designed an in vitro model system, involving genetic markers carried on two RNA templates, to allow a search for individual recombination events and to score their frequency of occurrence. We show that Moloney murine leukemia virus reverse transcriptase alone promotes homologous recombination efficiently. While RNA concentration has little effect on recombination frequency, there is a clear correlation between the amount of reverse transcriptase used in the assay and the extent of recombination observed. Under conditions mimicking the in vivo situation, a rate compatible with ex vivo estimates has been obtained.

引用

页码：6971 / 6975

页数：5

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