CERCOSPORA-BETICOLA TOXINS .7. FLUOROMETRIC STUDY OF THEIR INTERACTIONS WITH BIOLOGICAL-MEMBRANES

被引：15

作者：

MIKES, V ^{[1
]}

MILAT, ML ^{[1
]}

PUGIN, A ^{[1
]}

BLEIN, JP ^{[1
]}

机构：

[1] UNIV BOURGOGNE, INRA, UNITE ASSOCIEE PHYTOPHARM PHYTOBIOL CELLULAIRE, F-21034 DIJON, FRANCE

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 1994年 / 1195卷 / 01期

关键词：

BETICOLIN; LIPOSOME; PLASMA MEMBRANE; FLUORESCENCE; MAGNESIUM COMPLEX;

D O I：

10.1016/0005-2736(94)90018-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The interactions of two beticolins, Cercospora beticola toxins, and of their magnesium complexes with liposomes or plasma membrane were studied. The fluorometric pH titration curves of beticolins in liposomes and in plasma membranes reveal the presence of the dissociated form of beticolins. The concentration of the magnesium complex in these membranes increases at high pH. The partition coefficient of beticolin-1 on liposomes is 3-fold higher than that of beticolin-2 and the fluorescence of both compounds on liposomes is similar. The addition of magnesium to liposomes causes a 40-fold and 20-fold increase in the partition coefficient of beticolin-1 and -2, respectively, as a result of the interactions between membrane, magnesium and beticolins. Beticolins react to a Delta pH across the liposome membrane but the formation of the magnesium complex completely abolishes this effect.

引用

页码：124 / 130

页数：7

共 23 条

[1] SECONDARY MOLD METABOLITES .41. STRUCTURE AND BIOSYNTHESIS OF CERCOSPORA-BETICOLA TOXIN (CBT) [J].

ARNONE, A ;

NASINI, G ;

MERLINI, L ;

RAGG, E ;

ASSANTE, G .

JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1, 1993, (01) :145-151

[2] SCREENING OF GENUS CERCOSPORA FOR SECONDARY METABOLITES [J].

ASSANTE, G ;

LOCCI, R ;

CAMARDA, L ;

MERLINI, L ;

NASINI, G .

PHYTOCHEMISTRY, 1977, 16 (02) :243-247

[3] CERCOSPORA-BETICOLA TOXIN INHIBITS VANADATE-SENSITIVE H-+ TRANSPORT IN CORN ROOT MEMBRANE-VESICLES [J].

BLEIN, JP ;

BOURDIL, I ;

ROSSIGNOL, M ;

SCALLA, R .

PLANT PHYSIOLOGY, 1988, 88 (02) :429-434

[4]

Brand L., 1967, METHOD ENZYMOL, V87, P776, DOI [10.1016/S0076-6879(67)11091-4, DOI 10.1016/S0076-6879(67)11091-4]

[5] THE DELTA-PH-PROBE 9-AMINOACRIDINE - RESPONSE-TIME, BINDING BEHAVIOR AND DIMERIZATION AT THE MEMBRANE [J].

GRZESIEK, S ;

DENCHER, NA .

BIOCHIMICA ET BIOPHYSICA ACTA, 1988, 938 (03) :411-424

[6] CERCOSPORA-BETICOLA PHYTOTOXINS - CEBETINS THAT ARE PHOTOACTIVE, MG2+-BINDING, CHLORINATED ANTHRAQUINONE XANTHONE CONJUGATES [J].

JALAL, MAF ;

HOSSAIN, MB ;

ROBESON, DJ ;

VANDERHELM, D .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1992, 114 (15) :5967-5971

[7] PROBING BIOMEMBRANE INTERFACIAL POTENTIAL AND PH PROFILES WITH A NEW-TYPE OF FLOAT-LIKE FLUOROPHORES POSITIONED AT VARYING DISTANCE FROM THE MEMBRANE-SURFACE [J].

KRAAYENHOF, R ;

STERK, GJ ;

SANG, HWWF .

BIOCHEMISTRY, 1993, 32 (38) :10057-10066

[8]

KRAAYENHOF R, 1977, ELECTRICAL PHENOMENA, P493

[9]

Macri F., 1983, Phytopathologia Mediterranea, V22, P109

[10] INHIBITION OF K+ UPTAKE AND H+ EXTRUSION CAUSED BY NON-IRRADIATED CERCOSPORIN [J].

MACRI, F ;

VIANELLO, A .

PLANT SCIENCE LETTERS, 1981, 22 (01) :29-37

← 1 2 3 →