IMMUNOAFFINITY PURIFICATION OF AN OXIDASE-ACTIVATING CYTOSOLIC COMPLEX FROM BOVINE NEUTROPHILS

被引：12

作者：

JOUAN, A ^{[1
]}

DAGHER, MC ^{[1
]}

FUCHS, A ^{[1
]}

FOUCAUDGAMEN, J ^{[1
]}

VIGNAIS, PV ^{[1
]}

机构：

[1] UFR PHARM LA TRONCHE, MICROBIOL LAB, F-38706 LA TRONCHE, FRANCE

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 197卷 / 03期

关键词：

D O I：

10.1006/bbrc.1993.2618

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An oxidase activating complex from the cytosol of bovine neutrophils was purified by immunoaffinity using a monoclonal antibody specific for the 67 kDa cytosolic factor of oxidase activation (p67) and assayed for production of superoxide O−2 in a cell-free system. The complex comprised not only p67, but also the second cytosolic factor of 47 kDa (p47) in equivalent amounts. The p47-p67 complex showed a good oxidase activating potency when added to neutrophil membranes in the presence of GTP-γ-S and arachidonic acid. A ras-related small G protein could not be immunodetected in the p47-p67 activating complex, indicating that the GTP required for oxidase activation in the cell free system bound to a protein that was either present in catalytic amounts in the cytosolic complex or present in sufficient amount in the membrane fraction. © 1993 Academic Press. All rights reserved.

引用

页码：1296 / 1302

页数：7

共 22 条

[1]

ABO A, 1991, J BIOL CHEM, V266, P23577

[2]

ABO A, 1992, J BIOL CHEM, V267, P16767

[3] ACTIVATION OF THE NADPH OXIDASE INVOLVES THE SMALL GTP-BINDING PROTEIN P21RAC1 [J].

ABO, A ;

PICK, E ;

HALL, A ;

TOTTY, N ;

TEAHAN, CG ;

SEGAL, AW .

NATURE, 1991, 353 (6345) :668-670

[4]

ANDO S, 1992, J BIOL CHEM, V267, P25709

[5]

BABIOR BM, 1992, ADV ENZYMOL RAMB, V65, P49

[6] UNSATURATED FATTY-ACIDS STIMULATE NADPH-DEPENDENT SUPEROXIDE PRODUCTION BY CELL-FREE SYSTEM DERIVED FROM MACROPHAGES [J].

BROMBERG, Y ;

PICK, E .

CELLULAR IMMUNOLOGY, 1984, 88 (01) :213-221

[7]

BROMBERG Y, 1985, J BIOL CHEM, V260, P3539

[8]

DORSEUIL O, 1992, J BIOL CHEM, V267, P20540

[9]

GABIG TG, 1990, J IMMUNOL, V145, P945

[10] ACTIVATION OF A NADPH OXIDASE FROM HORSE POLYMORPHONUCLEAR LEUKOCYTES IN A CELL-FREE SYSTEM [J].

HEYNEMAN, RA ;

VERCAUTEREN, RE .

JOURNAL OF LEUKOCYTE BIOLOGY, 1984, 36 (06) :751-759

← 1 2 3 →