EFFECTS OF CHOLIC-ACID AND PROTECTAID FORMULATIONS ON HUMAN SPERM MOTILITY AND ULTRASTRUCTURE

被引:19
作者
COURTOT, AM
NIKAS, G
GRAVANIS, A
PSYCHOYOS, A
机构
[1] UNIV PARIS 11,UFR KREMLIN BICETRE,REPROD BIOL LAB,F-94270 LE KREMLIN BICETR,FRANCE
[2] UNIV CRETE,SCH MED,DEPT PHARMACOL,GR-71110 IRAKLION,GREECE
[3] HOP BICETRE,INSERM,F-94276 LE KREMLIN BICETR,FRANCE
关键词
CHOLIC ACID; BARRIER CONTRACEPTIVES; CONTRACEPTIVE; HUMAN SPERM ULTRASTRUCTURE; SPERMICIDES; VAGINAL SPONGE;
D O I
10.1093/oxfordjournals.humrep.a138382
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
Cholic acid (sodium cholate) and the other active ingredients of F-5 gel preparations in use for the impregnation of a new vaginal sponge (Protectaid(R)) with contraceptive and anti-sexually transmitted disease properties, were assessed for their effects on human sperm motility and ultrastructure. Cholic acid (CA) produced an inhibition of motility which was both dose- and time-dependent. A complete suppression of motility was obtained at 30 s by a CA concentration of 1.25%. Nonoxynol-9 (NX9) compared with benzalkonium chloride (BZC) showed no significant difference at the concentration required (0.025%) to give a total inhibition of sperm motility after exposure for 30 s. The addition of F-5A gel containing 0.5% of each one of the spermicide ingredients (CA, NX9 and BZC) produced the total suppression of sperm motility within 30 s at a dilution of 1/50. Another preparation, F-5B gel, containing the spermicide ingredients at different concentrations (1.25% CA, 0.125% NX9 and 0.05% BZC) produced this same effect with a 1/10 dilution. Exposure of semen to a CA concentration of 1.25% or to 1/10 dilutions of F-5A gel for 30 s led to profound changes of sperm ultrastructure studied by scanning (SEM) and transmission (TEM) electron microscopy. SEM and TEM findings indicate that CA acts as a spermicide through its 'natural detergent' properties, damaging the outer plasma membrane of sperm cells. Protectaid formulations affect sperm motility and viability in a similar way.
引用
收藏
页码:1999 / 2005
页数:7
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