PRELIMINARY CRYSTALLOGRAPHIC STUDY OF PROTOCATECHUATE 3,4-DIOXYGENASE FROM BREVIBACTERIUM-FUSCUM

被引：15

作者：

EARHART, CA ^{[1
]}

RADHAKRISHNAN, R ^{[1
]}

ORVILLE, AM ^{[1
]}

LIPSCOMB, JD ^{[1
]}

OHLENDORF, DH ^{[1
]}

机构：

[1] UNIV MINNESOTA,SCH MED,DEPT BIOCHEM,MINNEAPOLIS,MN 55455

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1994年 / 236卷 / 01期

关键词：

BREVIBACTERIUM FUSCUM; DIOXYGENASES; X-RAY CRYSTALLOGRAPHY; CRYSTALLIZATION;

D O I：

10.1006/jmbi.1994.1143

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The enzyme protocatechuate 3,4-dioxygenase from the Gram positive organism Brevibacterium fuscum crystallizes in the triclinic space group P1 with unit cell dimensions a = 96.1 Å, b = 97.2 Å, c = 118.1 Å and α = 113.9°, β = 90.7°, γ = 117.8°. The rod-like crystals diffract to 2.4 Å resolution. Rotation function analysis suggests that there are six protomers arranged with local 32 symmetry in the asymmetric unit rather than the previously proposed pentameric complex. © 1994 Academic Press Limited.

引用

页码：374 / 376

页数：3

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