ISOLATION AND SEQUENCE OF THE TRANSFER-RNA LIGASE-ENCODING GENE OF CANDIDA-ALBICANS

被引：13

作者：

BAYMILLER, J

JENNINGS, S

KIENZLE, B

GORMAN, JA

KELLY, R

MCCULLOUGH, JE

机构：

[1] BRISTOL MYERS SQUIBB PHARMACEUT RES INST,DEPT MICROBIAL MOLEC BIOL,PRINCETON,NJ 08543

[2] BRISTOL MYERS SQUIBB PHARMACEUT RES INST,DEPT METAB DIS,PRINCETON,NJ 08543

来源：

GENE | 1994年 / 142卷 / 01期

关键词：

SACCHAROMYCES; NUCLEOTIDE SEQUENCE; PULSED-FIELD GEL ELECTROPHORESIS;

D O I：

10.1016/0378-1119(94)90367-0

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The gene encoding tRNA ligase from Candida albicans was isolated from a genomic library by complementation of a Saccharomyces cerevisiae strain containing a disrupted structural gene, RLG1, encoding tRNA ligase. The cloned gene also complements a temperature-sensitive allele of RLG1. Sequence analysis revealed a single 2499-nt coding region. The gene encodes a protein of 833 amino acids that is 42% identical to S. cerevisiae tRNA ligase. Hybridization to chromosomes of C. albicans separated by pulsed-field gel electrophoresis located the gene to chromosome 1, the smallest C. albicans chromosome.

引用

页码：129 / 134

页数：6

共 31 条

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BOEKE JD, 1987, METHOD ENZYMOL, V154, P164

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