A MODIFIED METHOD FOR THE INDIRECT QUANTITATIVE-ANALYSIS OF PHYTATE IN FOODSTUFFS

被引：93

作者：

FRUHBECK, G ^{[1
]}

ALONSO, R ^{[1
]}

MARZO, F ^{[1
]}

SANTIDRIAN, S ^{[1
]}

机构：

[1] PUBL UNIV NAVARRA,ANIM PHYSIOL & NUTR LAB,E-31006 PAMPLONA,SPAIN

来源：

ANALYTICAL BIOCHEMISTRY | 1995年 / 225卷 / 02期

关键词：

D O I：

10.1006/abio.1995.1145

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple, precise, and reproducible method to quantitatively assess total phytate content in foodstuffs has been developed. After HCl extraction, samples were run through an anion-exchange column and total phytate content of the eluates was spectrophotometrically measured with the modified Wade reagent. The main changes introduced in our procedure refer to pH adjustment in the extraction, dilution, and quantitation steps (pH 0.60, 6.00, and 3.00, respectively). Furthermore, the minimal extraction time for legumes (2 h) was determined. In addition, the effect of protein content on phytate determination was tested. Phytate content directly measured in crude extracts omitting anion-exchange chromatographic purification was significantly lower (P < 0.01) than values obtained using column separation, thus demonstrating that reliable phytate assessment in foodstuffs demands the utilization of anion-exchange chromatography. (C) 1995 Academic Press, Inc.

引用

页码：206 / 212

页数：7

共 27 条

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