TRANSGENIC MALLING-26 APPLE EXPRESSING THE ATTACIN-E GENE HAS INCREASED RESISTANCE TO ERWINIA-AMYLOVORA

被引：74

作者：

NORELLI, JL ^{[1
]}

ALDWINCKLE, HS ^{[1
]}

DESTEFANOBELTRAN, L ^{[1
]}

JAYNES, JM ^{[1
]}

机构：

[1] LOUISIANA STATE UNIV,DEPT BIOCHEM,BATON ROUGE,LA 70803

来源：

EUPHYTICA | 1994年 / 77卷 / 1-2期

关键词：

APPLE; ATTACIN; TRANSFORMATION; RESISTANCE; FIRE BLIGHT; ERWINIA AMYLOVORA; MALUS DOMESTICA;

D O I：

10.1007/BF02551474

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Apple (Malus domestica) transgenic T1 was obtained by Agrobacterium tumefaciens-mediated transformation of Malling 26 rootstock using the plasmid binary vector pLDB15.pLDB15 contains within its T-DNA a gene encoding the lytic protein attacin E. The integration of the attacin E gene into the apple genome was confirmed by Southern analysis. Northern analysis indicated the presence of an attacin E mRNA in plants inoculated with Erwinia amylovora. After inoculation of in vitro grown plants of T1, Malling 26, and Malling 7 (resistant control) with E amylovora, the log(10) of the inoculum concentration lethal to 50% of the plants was 5.4, 4.4, and 5.6, respectively. In greenhouse trials for resistance to fire blight, T1 was significantly more resistant than 'Malling 26'.

引用

页码：123 / 128

页数：6

共 21 条

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