EVALUATION OF METHODS FOR THE ISOLATION AND DETECTION OF ESCHERICHIA-COLI O157 IN MINCED BEEF

被引：41

作者：

BENNETT, AR

MACPHEE, S

BETTS, RP

机构：

[1] Campden and Chorleywood Food Research Association, Chipping Campden

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1995年 / 20卷 / 06期

关键词：

D O I：

10.1111/j.1472-765X.1995.tb01325.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

This study has evaluated enrichment and detection procedures for the isolation and detection of Escherichia coli O157 inoculated into minced beef. The use of a 24 h enrichment in modified EC broth containing novobiocin allowed low numbers of contaminating cells to multiply to levels detectable on culture media and by ELISA test kits. Total analysis time was reduced by the use of the Dynabead(TM) immunomagnetic separation system. The use of the Petrifilm(TM) Test Kit-HEC for E. coli O157:H7 and Organon Teknika EHEC-TEK system detected low numbers of contaminating cells following enrichment and reduced analysis time by 1 d. The incorporation of cefixime and tellurite into Sorbitol MacConkey Agar increased the rate and ease of isolation off. coli O157 and its use is therefore recommended.

引用

页码：375 / 379

页数：5

共 17 条

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