RECOMBINANT WHOLE-CELL PENICILLIN ACYLASE BIOCATALYST - PRODUCTION, CHARACTERIZATION AND USE IN THE SYNTHESIS AND HYDROLYSIS OF ANTIBIOTICS

被引：7

作者：

OSPINA, S

MERINO, E

RAMIREZ, OT

LOPEZMUNGUIA, A

机构：

[1] UNIV NACL AUTONOMA MEXICO,INST BIOTECNOL,CUERNAVACA 62271,MORELOS,MEXICO

[2] UNIV NACL COLOMBIA,INST BIOTECNOL,DEPT FARM,BOGOTA 14490,COLOMBIA

[3] UNIV NACL AUTONOMA MEXICO,INST BIOTECNOL,CUERNAVACA 62271,MORELOS,MEXICO

来源：

BIOTECHNOLOGY LETTERS | 1995年 / 17卷 / 06期

关键词：

D O I：

10.1007/BF00129388

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The production of a whole cell biocatalyst with high specific penicillin acylase activity using a recombinant strain is described High activities were obtained using exponentially fed-batch fermentations to overproduce the enzyme. The cells were then entrapped in agar and the activity characterized in terms of particle size and,stability. The technical feasibility of the catalyst in synthesis and hydrolysis reactions was demonstrated.

引用

页码：615 / 620

页数：6

共 12 条

[1]

GOMEZ A, 1994, PROCESS COMPUTATIONS

[2] PURIFIED PENICILLIN ACYLASE PERFORMANCE IN A STABILIZED ULTRAFILTRATION MEMBRANE REACTOR [J].