IMMUNOFRACTIONATION OF DNA-SEQUENCES ASSOCIATED WITH HMG-17 IN CHROMATIN

被引：45

作者：

DRUCKMANN, S ^{[1
]}

MENDELSON, E ^{[1
]}

LANDSMAN, D ^{[1
]}

BUSTIN, M ^{[1
]}

机构：

[1] NCI, MOLEC CARCINOGENESIS LAB, BLDG 37, ROOM 3D12, BETHESDA, MD 20892 USA

来源：

EXPERIMENTAL CELL RESEARCH | 1986年 / 166卷 / 02期

关键词：

D O I：

10.1016/0014-4827(86)90493-3

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Antibodies specific for chromosomal protein HMG-17 were immoblilized on CNBr-Sepharose and the resulting immunoaffinity column was used to purify chromatin segments enriched in HMG-17. The DNA was purified from both the nucleosomal fraction which was bound to the column and from the fraction which was not bound, and examined with DNA probes representing repetitive DNA, non-transcribed genes, transcribed genes and inducible genes. The results suggest that HMG-17 is preferentially associated with DNA sequences coding for genes, regardless of whether they are transcribed, and therefore support the notion that HMG-17 confers specific structural characteristics on selected regions in the genome.

引用

页码：486 / 496

页数：11

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