SIMPLE AND RAPID PROCEDURE FOR THE ANALYSIS OF REDUCING, TOTAL AND INDIVIDUAL SUGARS IN POTATOES

被引:37
作者
CRONIN, DA
SMITH, S
机构
[1] Department of Agricultural Chemistry and Soil Science, Faculty of Agriculture, University College, Dublin 4, Belfield
关键词
glucose oxidase; inversion; micro-colorimetric method; Somogi; sucrose;
D O I
10.1007/BF02366940
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
A simple analytical scheme based on the micro-colorimetric reducing sugar methods of Somogi is described which permits the reducing and total sugars, as well as glucose, fructose and sucrose, to be determined in aqueous methanolic extracts of potatoes. The reducing and total sugars reskpectively-and thus, by difference, sucrose - are determined by estimating reducing power before and after inversion with hot dilute HCl. The individual reducing sugars are estimated from the decrease in reducing power after complete removal of glucose with glucose oxidase. The procedure is rapid and convenient and permits estimations to be made to within approximately ±5% of actual values. © 1979 Kluwer Academic Publishers.
引用
收藏
页码:99 / 105
页数:7
相关论文
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