CLONING OF TRICHOSANTHIN CDNA AND ITS EXPRESSION IN ESCHERICHIA-COLI

被引：62

作者：

SHAW, PC ^{[1
]}

YUNG, MH ^{[1
]}

ZHU, RH ^{[1
]}

HO, WKK ^{[1
]}

NG, TB ^{[1
]}

YEUNG, HW ^{[1
]}

机构：

[1] CHINESE UNIV HONG KONG,BIOTECHNOL LAB,SHA TIN,HONG KONG

来源：

GENE | 1991年 / 97卷 / 02期

关键词：

RECOMBINANT DNA; ROOT TUBER; SIGNAL SEQUENCE; NUCLEOTIDE SEQUENCE; EXPRESSION;

D O I：

10.1016/0378-1119(91)90061-F

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Several cDNA clones coding for trichosanthin (TCS) have been isolated from a cDNA library prepared from the poly(A)+RNA of the root tuber of Trichosanthes kirilowii Maximowicz. The nucleotide sequence codes for a protein of 289 amino acids (aa) including a putative signal peptide of 23 aa and an extra 19 aa at the C terminus; the latter two have not been found in TCS obtained from the natural product [Collins et al., J. Biol. Chem. 265 (1990) 8665-8669]. Recombinant TCS (reTCS) was synthesized in Escherichia coli, in which the cDNA without the signal sequence was expressed under the control of the trc promoter; reTCS was detected by a rabbit anti-TCS antiserum.

引用

页码：267 / 272

页数：6

共 28 条

[1] TIGHTLY REGULATED TAC PROMOTER VECTORS USEFUL FOR THE EXPRESSION OF UNFUSED AND FUSED PROTEINS IN ESCHERICHIA-COLI [J].

AMANN, E ;

OCHS, B ;

ABEL, KJ .

GENE, 1988, 69 (02) :301-315

[2]

BJERRUM O, 1986, ANCOS GUIDE SEMIDRY

[3] TRICHOKIRIN, A RIBOSOME-INACTIVATING PROTEIN FROM THE SEEDS OF TRICHOSANTHES-KIRILOWII MAXIMOWICZ - PURIFICATION, PARTIAL CHARACTERIZATION AND USE FOR PREPARATION OF IMMUNOTOXINS [J].

CASELLAS, P ;

DUSSOSSOY, D ;

FALASCA, AI ;

BARBIERI, L ;

GUILLEMOT, JC ;

FERRARA, P ;

BOLOGNESI, A ;

CENINI, P ;

STIRPE, F .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1988, 176 (03) :581-588

[4] THE TERMINATION OF EARLY-PREGNANCY IN THE MOUSE BY BETA-MOMORCHARIN [J].

CHAN, WY ;

TAM, PPL ;

YEUNG, HW .

CONTRACEPTION, 1984, 29 (01) :91-100

[5] SUPERCOIL SEQUENCING - A FAST AND SIMPLE METHOD FOR SEQUENCING PLASMID DNA [J].

CHEN, EY ;

SEEBURG, PH .

DNA-A JOURNAL OF MOLECULAR & CELLULAR BIOLOGY, 1985, 4 (02) :165-170

[6]

CHOW PT, 1990, J BIOL CHEM, V265, P8670

[7]

COLLINS EJ, 1990, J BIOL CHEM, V265, P8665

[8] RIBONUCLEIC-ACID ISOLATED BY CESIUM-CHLORIDE CENTRIFUGATION [J].

GLISIN, V ;

CRKVENJAKOV, R ;

BYUS, C .

BIOCHEMISTRY, 1974, 13 (12) :2633-2637

[9]

HARLOW E, 1988, ANTIBODIES LABORATOR

[10]

HUANG Y, 1987, CHIN J INTEGRAT TRAD, V7, P154

← 1 2 3 →