COMPARISON OF PERCOLL, MINI-PERCOLL AND SWIM-UP METHODS FOR SPERM PREPARATION FROM ABNORMAL SEMEN SAMPLES

被引：87

作者：

NG, FLH ^{[1
]}

LIU, DY ^{[1
]}

BAKER, HWG ^{[1
]}

机构：

[1] UNIV MELBOURNE,DEPT OBSTET & GYNAECOL,MELBOURNE,VIC 3053,AUSTRALIA

来源：

HUMAN REPRODUCTION | 1992年 / 7卷 / 02期

关键词：

MALE INFERTILITY; SPERM PREPARATION; IVF;

D O I：

10.1093/oxfordjournals.humrep.a137628

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Two methods of density gradient centrifugation, Percoll (P) and mini-Percoll (MP), were compared with the swim-up technique for preparing spermatozoa from each of 40 abnormal semen samples. P and MP produced similar results with a mean recovery of spermatozoa with progressive motility which was significantly higher (18-19%) than that achieved with swim-up (5%). However, the swim-up method resulted in the recovery of spermatozoa with a higher mean motility (89 versus 58%), velocity (69 versus 56-mu-m/s), percentage with normal morphology (22 versus 16%) and intact acrosomes (61 versus 36%) than P and MP. The mean amplitude of lateral head displacement was the only characteristic of spermatozoa in semen which correlated with the recoveries of motile spermatozoa. Combining MP and swim-up methods for 10 samples produced a higher recovery (11 versus 6.9%) of spermatozoa with significantly better mean motility (94 versus 87%) than did swim-up alone. Although P and MP resulted in greater yields of motile spermatozoa than the swim-up preparation, the latter procedure selected higher proportions of spermatozoa with improved characteristics (velocity, intact acrosomes and normal morphology) which correlate with fertilization rates in vitro. It is concluded that P and MP are not superior to swim-up. However, sequential MP and swim-up preparation improves yields of high quality spermatozoa from some abnormal semen samples and therefore has potential for improving fertilization rates.

引用

页码：261 / 266

页数：6

共 37 条

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