CHEMILUMINESCENCE DETECTION OF 8A-HYDROPEROXY-TOCOPHERONE IN PHOTOOXIDIZED ALPHA-TOCOPHEROL

被引：8

作者：

MIYAZAWA, T

YAMASHITA, T

FUJIMOTO, K

机构：

[1] Department of Food Chemistry, Tohoku University, Sendai, 981, Tsutsumidori Amamiyamachi 1-1, Aoba-ku

来源：

LIPIDS | 1992年 / 27卷 / 04期

关键词：

D O I：

10.1007/BF02536478

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Photosensitized oxidation products of alpha-tocopherol were analyzed by reversed phase high-performance liquid chromatography (CL-HPLC) in combination with chemiluminescence detection, to measure hydroperoxide derivatives. An intense peak was observed on the chemiluminescence chromatogram, corresponding to 8a-hydroperoxy-alpha-tocopherone (alpha-tocopherol hydroperoxide), the identity of which was confirmed by H-1 nuclear magnetic resonance and mass spectrometry. Observed peak intensities correlated with the peroxide value of oxidized alpha-tocopherol. As little as 50 pmol of 8a-hydroperoxy-alpha-tocopherone was detectable. The CL-HPLC method, which is specific for the detection of hydroperoxides, should prove useful in studies on tocopherol oxidation in foods and biological systems.

引用

页码：289 / 294

页数：6

共 33 条

[1] ON THE OCCURRENCE OF ALPHA-TOCOPHEROLQUINONE IN RAT-TISSUE [J].