HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF ETHACRYNIC-ACID IN HUMAN PLASMA

被引：6

作者：

LACRETA, FP ^{[1
]}

BRENNAN, JM ^{[1
]}

TINSLEY, PW ^{[1
]}

ODWYER, PJ ^{[1
]}

机构：

[1] FOX CHASE CANC INST,DEPT MED,DEPT DEV CHEMOTHERAPY,PHILADELPHIA,PA 19111

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 571卷 / 1-2期

关键词：

D O I：

10.1016/0378-4347(91)80455-L

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A high-performance liquid chromatographic method for the determination of ethacrynic acid (EA) in human plasma is described. Plasma was prepared for analysis by addition of 4-(2,4-dichlorophenoxy)-butyric acid as an internal standard followed by acidification with hydrochloric acid and extraction with ethyl acetate. Separation was by isocratic reversed-phase chromatography, the column effluent was monitored at 280 nm and quantitation was performed using peak-area ratios. The linear range for EA determination was from 0.5 to 25-mu-g/ml with a lower limit of detection of 0.1-mu-g/ml. The reported method is convenient, sensitive and reproducible, illustrating its usefulness for pharmacokinetic studies.

引用

页码：271 / 276

页数：6

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