GENOTYPING OF STREPTOCOCCUS-THERMOPHILUS EVIDENCED BY RESTRICTION ANALYSIS OF RIBOSOMAL DNA

被引：18

作者：

SALZANO, G

MOSCHETTI, G

VILLANI, F

PEPE, O

MAURIELLO, G

COPPOLA, S

机构：

[1] UNIV NAPLES FEDERICO II, IST MICROBIOL AGR, I-80055 PORTICI, ITALY

[2] UNIV NAPLES FEDERICO II, MICROBIOL IND STN, I-80055 PORTICI, ITALY

[3] UNIV MOLISE, DISTAAM, I-86100 CAMPOBASSO, ITALY

来源：

RESEARCH IN MICROBIOLOGY | 1994年 / 145卷 / 09期

关键词：

RIBOTYPING; STREPTOCOCCUS THERMOPHILUS; FOOD; RDNA; RIBOSOMAL-RNA; ARDRA PCR; DIAGNOSIS; TAXONOMY;

D O I：

10.1016/0923-2508(94)90036-1

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Twenty strains of Streptococcus thermophilus were classified into five different types characterized by ribotyping after DNA digestion with HindIII and hybridization with cDNA from 16S-23S rDNA of Escherichia coli. Ribotyping after digestion with HaeIII and XhoI enabled the same strains to be gathered into three groups as a consequence of the reduced influence of the flanking sequences within the analysis of rRNA operons. Amplified rDNA restriction analysis clearly confirmed the discrimination of these three different ribotypes, producing evidence to suggest that subtaxa are to be recognized within this species.

引用

页码：651 / 658

页数：8

共 20 条

[1] RAPID GENOMIC FINGERPRINTING OF LACTOCOCCUS-LACTIS STRAINS BY ARBITRARILY PRIMED POLYMERASE CHAIN-REACTION WITH P-32 AND FLUORESCENT LABELS [J].