ELUTION OF HUMAN-IGG FROM AFFINITY COLUMNS CONTAINING IMMOBILIZED VARIANTS OF PROTEIN-A

被引:19
作者
BOTTOMLEY, SP
SUTTON, BJ
GORE, MG
机构
[1] UNIV SOUTHAMPTON,INST BIOMOLEC SCI,DEPT BIOCHEM,SOUTHAMPTON SO16 7PX,HANTS,ENGLAND
[2] UNIV LONDON KINGS COLL,RANDALL INST,DIV BIOMED SCI,LONDON WC2B 5RL,ENGLAND
关键词
CHROMATOGRAPHY; AFFINITY; IGG; IMMUNOGLOBULIN BINDING; PROTEIN A;
D O I
10.1016/0022-1759(95)00049-G
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Immobilised analogues of protein A have been used for affinity chromatographic separation of human IgG. Truncation of the C-terminal region of an engineered IgG-binding domain based upon the B domain from protein A, in combination with site-directed mutagenesis, has led to the generation of a number of proteins with a decreased affinity for IgG. The elution of human IgG from these proteins when immobilised onto a solid support occurs over the pH range 3.2-5.0 with 0.5 M acetate buffer. These proteins may be effective alternatives to standard protein A columns when milder elution conditions are required.
引用
收藏
页码:185 / 192
页数:8
相关论文
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