CRY IA(B) TRANSCRIPT FORMATION IN TOBACCO IS INEFFICIENT

被引：49

作者：

VANAARSSEN, R ^{[1
]}

SOETAERT, P ^{[1
]}

STAM, M ^{[1
]}

DOCKX, J ^{[1
]}

GOSSELE, V ^{[1
]}

SEURINCK, J ^{[1
]}

REYNAERTS, A ^{[1
]}

CORNELISSEN, M ^{[1
]}

机构：

[1] PLANT GENET SYST NV,B-9000 GHENT,BELGIUM

来源：

PLANT MOLECULAR BIOLOGY | 1995年 / 28卷 / 03期

关键词：

CRY GENES; MESSENGER-RNA STABILITY; RNA SPLICING;

D O I：

10.1007/BF00020398

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Chimaeric P(CaMV35S)cry genes direct in tobacco mesophyll protoplasts mRNA levels of less than one transcript per cell. We provide evidence that this low cytoplasmic cryIA(b) mRNA level is not due to a rapid turnover but rather results from a marginal import flow of cry messenger into the cytoplasm. Run-on assays indicate that the frequency of transcription initiation is not limiting. However, the cry precursor mRNA carries at least three regions that are recognized as introns. The absence of high cytoplasmic levels of spliced cry mRNAs suggests that these mRNAs are unstable and/or not efficiently made. Point mutations in the 5' splice site of the most distal intron allows high accumulation levels of the full-length mRNA. This implies that the inefficient formation of full-size mRNA is a major cause of the low expression level of chimaeric cry IA(b) genes in tobacco.

引用

页码：513 / 524

页数：12

共 46 条

[1]

NUCLEASE ACTIVITY ASSOCIATED WITH MAMMALIAN MESSENGER-RNA IN ITS NATIVE-STATE - POSSIBLE BASIS FOR SELECTIVITY IN MESSENGER-RNA DECAY [J].