VARIATION IN TOTAL SOLUBLE SUGARS OF CONIFER TISSUES WITH METHOD OF ANALYSIS

被引:114
作者
EBELL, LF
机构
[1] Forest Research Laboratory, Canada Department of Forestry and Rural Development, Victoria
关键词
D O I
10.1016/S0031-9422(00)85818-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Techniques are described for sample preparation and analysis of total ethanol-soluble sugars of conifer tissue, using a stabilized, diluted anthrone reagent. This reagent gave similar values for the principla free sugars, in contrast to a greater divergence with other anthrone reagents and with the phenol-sulphuric method. Non-carbohydrate interfering substances caused serious overestimations of the sugar content of fully cleared samples by a reducing sugar method. Deproteinizing was not required for colorimetric methods but decolorizing with charcoal was essential, largely to remove interfering phenolic compounds. © 1970.
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页码:227 / &
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