A NOVEL FLOW CYTOMETRIC METHOD FOR MEASURING PROTEIN DIGESTION WITHIN THE PHAGOCYTIC VACUOLE OF POLYMORPHONUCLEAR NEUTROPHILS

被引：4

作者：

HAYNES, AP ^{[1
]}

FLETCHER, J ^{[1
]}

GARNETT, M ^{[1
]}

ROBINS, A ^{[1
]}

机构：

[1] UNIV NOTTINGHAM,CANC RES CAMPAIGN LABS,NOTTINGHAM NG7 2RD,ENGLAND

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1990年 / 135卷 / 1-2期

关键词：

NEUTROPHIL; PROTEIN DIGESTION; RHODAMINE; FLOW CYTOMETRY;

D O I：

10.1016/0022-1759(90)90268-Z

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

When rhodamine is attached to albumin at a high molar ratio its fluorescence is quenched but fluorescence is released when the protein is digested and the dye released. Using the observation it is possible to measure protein digestion within the phagocytic vacuole of neutrophils. The assay is simple, rapid and measures digestion even in the presence of abnormal phagocytosis.

引用

页码：155 / 161

页数：7

共 8 条

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