EXTRINSIC LABELING OF CASEINOPHOSPHOPEPTIDES WITH CA-45 AND RECOVERY FOLLOWING THERMAL-TREATMENT

被引:3
作者
KITTS, DD
LEUNG, R
NAKAI, S
机构
来源
CANADIAN INSTITUTE OF FOOD SCIENCE AND TECHNOLOGY JOURNAL-JOURNAL DE L INSTITUT CANADIEN DE SCIENCE ET TECHNOLOGIE ALIMENTAIRES | 1991年 / 24卷 / 05期
关键词
D O I
10.1016/S0315-5463(91)70165-X
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
The determination and degree of extrinsic labelling of tryptic derived caseinophosphopeptide with Ca-45 was performed in native and heat treated whole casein and skim milk protein powders. Two principal phosphopeptides, alpha(2)2 (1-21) and beta-casein (1-25) were identified by peptide sequencing following isolation by HPLC. These particular peptides accounted for similar Ca-45 binding activities in the crude CPP fraction. The application of a thermal process to casein and skim milk protein powder resulted in substantial dephosphorylation, but had little effect on both the yield and degree of extrinsic labelling of caseinophosphopeptides by Ca-45.
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页码:278 / 282
页数:5
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