COMPOSITION ANALYSIS OF CARBOXYMETHYLCELLULOSE BY HIGH-PH ANION-EXCHANGE CHROMATOGRAPHY WITH PULSED AMPEROMETRIC DETECTION

被引：53

作者：

KRAGTEN, EA ^{[1
]}

KAMERLING, JP ^{[1
]}

VLIEGENTHART, JFG ^{[1
]}

机构：

[1] UNIV UTRECHT,DEPT BIOORGAN CHEM,CTR BIJVOET,POB 80075,3508 TB UTRECHT,NETHERLANDS

来源：

JOURNAL OF CHROMATOGRAPHY | 1992年 / 623卷 / 01期

关键词：

D O I：

10.1016/0021-9673(92)85297-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A rapid method for the determination of the substituent distribution in carboxymethylcellulose has been developed, involving hydrolysis of carboxymethylcellulose in 1.2 M perchloric acid and analysis of the mixture of carboxymethylated glucose residues and glucose by high-pH anion-exchange chromatography with pulsed amperometric detection. The peaks in the chromatogram were identified by combined gas-liquid chromatography-mass spectrometry after pertrimethylsilylation. Molar response factors for each of the constituent monomers were established by H-1 NMR spectroscopy. The degrees of substitution for three carboxymethylcellulose preparations determined by the proposed method and by a standard titration method were found to be in excellent agreement.

引用

页码：49 / 53

页数：5

共 17 条

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