THE EFFECT OF CERTAIN SOIL TREATMENTS ON DIDYMELLA STEM-ROT OF TOMATOES II. LABORATORY INVESTIGATIONS

About 93 % of Didymella lycopersiei spores were destroyed after 4 weeks incubation in unsterilized soil. A survey of the microflora of glasshouse soil receiving different treatments and inoculated with D. lycopersici showed no clear relation between numbers of any group of organisms and the incidence of stem rot. Sterilized soil was not made toxic to D. lycopersici by the growth of a number of soil micro-organisms even after 9 months incubation, but addition of unsterilized soil or of a suspension of unsterilized soil quickly restored toxicity. Direct observations of spores in soil on slides showed that their fate varied with the treatment of the soil before inoculation. With fresh soil or air-dry soil moistened z or more days before inoculation, lysis of spores occurred. With air-dry soil moistened and inoculated simultaneously, some spores germinated but growth of germ tubes soon ceased. No direct connexion could be seen between the fate of the spores and soil micro-organisms. Addition of glucose to unsterilized soil reduced its toxicity to D. lycoperski. Soils steamed for I min. or longer were not toxic to D. lycopersici, but soils steamed for very short periods were as toxic as unsterilized soils although the soil microflora was much reduced.