THE RESPONSE OF HUMAN DERMAL MICROVASCULAR ENDOTHELIAL-CELLS TO HYPOXIA

被引:3
作者
MANSBRIDGE, J
MURPHY, B
MORHENN, V
HILLMAN, K
HILLMAN, K
SUTHERLAND, R
KARASEK, M
机构
[1] STANFORD UNIV,SCH MED,DEPT DERMATOL,STANFORD,CA 94305
[2] SRI INT,DEPT CELL & MOLEC BIOL,MENLO PK,CA 94025
来源
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 1994年 / 1223卷 / 02期
关键词
MICROVASCULAR; ENDOTHELIUM; HYPOXIA; GENE EXPRESSION; OXYGEN REGULATED PROTEIN (ORP);
D O I
10.1016/0167-4889(94)90228-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Dermal microvascular endothelial cells (DMEC) exposed to hypoxic conditions show a rapid induction of several proteins that do not increase in other cell types placed in a similar environment. These DMEC proteins differ from the well-characterized stress proteins that have been observed in a wide variety of cultured cell types. The DMEC proteins are induced rapidly, within 2-4 h, and are expressed transiently. They include a group of acidic proteins (pI similar to 5-5.2) with molecular weights in the range 100 000-120 000 and at least one glycoprotein (pI 5.1, M(r) 57 000) that is probably expressed on the cell surface. In some primary DMEC cell strains, this response is accompanied by a transient overall increase in protein synthesis. The oxygen-regulated proteins (ORP) that are induced in most other cell types under hypoxic conditions show little variation in their rate of synthesis in DMEC within the first 24 h. The response of DMEC differs from that of umbilical vein endothelial cells (UVEC) and from spindle-shaped cells derived from DMEC, that show a response to hypoxia that is similar to most other cell types. The changes seen in DMEC proteins take place in the same time scale as ischemia-reperfusion injury and may reflect the specialized change of functions of the microvasculature observed under conditions of hypoxic stress in vivo.
引用
收藏
页码:209 / 218
页数:10
相关论文
共 37 条
[1]   THE MAJOR ANOXIC STRESS RESPONSE PROTEIN-P34 IS A DISTINCT LACTATE-DEHYDROGENASE [J].
ANDERSON, GR ;
FARKAS, BK .
BIOCHEMISTRY, 1988, 27 (06) :2187-2193
[2]  
AUCOIN MM, 1991, FASEB J, V5, pA1618
[3]   EXPRESSION OF ORGAN-SPECIFIC ANTIGENS ON CAPILLARY ENDOTHELIAL-CELLS [J].
AUERBACH, R ;
ALBY, L ;
MORRISSEY, LW ;
TU, M ;
JOSEPH, J .
MICROVASCULAR RESEARCH, 1985, 29 (03) :401-411
[4]   ISOLATION AND GROWTH OF ENDOTHELIAL-CELLS FROM THE MICRO-VESSELS OF THE NEWBORN HUMAN FORESKIN IN CELL-CULTURE [J].
DAVISON, PM ;
BENSCH, K ;
KARASEK, MA .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1980, 75 (04) :316-321
[5]  
DAVISON PM, 1983, IN VITRO CELL DEV B, V19, P937
[6]   HUMAN DERMAL MICRO-VASCULAR ENDOTHELIAL-CELLS INVITRO - EFFECT OF CYCLIC-AMP ON CELLULAR MORPHOLOGY AND PROLIFERATION RATE [J].
DAVISON, PM ;
KARASEK, MA .
JOURNAL OF CELLULAR PHYSIOLOGY, 1981, 106 (02) :253-258
[7]   PROTEIN-KINASE-C ACTIVATORS SUPPRESS STIMULATION OF CAPILLARY ENDOTHELIAL-CELL GROWTH BY ANGIOGENIC ENDOTHELIAL MITOGENS [J].
DOCTROW, SR ;
FOLKMAN, J .
JOURNAL OF CELL BIOLOGY, 1987, 104 (03) :679-687
[8]  
DOWNEY J, 1986, CIRCULATION, V74, P372
[9]   FREE RADICAL-PRODUCING ENZYME, XANTHINE-OXIDASE, IS UNDETECTABLE IN HUMAN HEARTS [J].
EDDY, LJ ;
STEWART, JR ;
JONES, HP ;
ENGERSON, TD ;
MCCORD, JM ;
DOWNEY, JM .
AMERICAN JOURNAL OF PHYSIOLOGY, 1987, 253 (03) :H709-H711
[10]   SPECIAL REPORT - THE COMPLEXITY OF ENDOTHELIAL-CELLS [J].
FAJARDO, LF .
AMERICAN JOURNAL OF CLINICAL PATHOLOGY, 1989, 92 (02) :241-250