LOCALIZATION OF CHOLINE ACETYLTRANSFERASE-EXPRESSING NEURONS IN THE LARVAL VISUAL-SYSTEM OF DROSOPHILA-MELANOGASTER

被引:45
作者
YASUYAMA, K [1 ]
KITAMOTO, T [1 ]
SALVATERRA, PM [1 ]
机构
[1] CITY HOPE NATL MED CTR, BECKMAN RES INST, DIV NEUROSCI, DUARTE, CA 91010 USA
关键词
CHOLINE ACETYLTRANSFERASE; CHOLINERGIC NEURON; VISUAL SYSTEM; BOLWIGS ORGAN; IMMUNOCYTOCHEMISTRY; IN SITU HYBRIDIZATION; DROSOPHILA MELANOGASTER (INSECTA);
D O I
10.1007/BF00319111
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Choline acetyltransferase (ChAT) is the enzyme catalyzing the biosynthesis of acetylcholine and is considered to be a phenotypically specific marker for cholinergic neurons. We have examined the distribution of ChAT-expressing neurons in the larval nervous system of Drosophila melanogaster by three different but complementary techniques: in situ hybridization with a cRNA probe to ChAT messenger RNA, immunocytochemistry using a monoclonal anti-ChAT antibody, and X-gal staining of transformed animals carrying a reporter gene composed of 7.4 kb of 5' flanking DNA from the ChAT gene fused to a lacZ reporter gene. All three techniques demonstrated ChAT-expressing neurons in the larval visual system. In embryos, the photoreceptor organ (Bolwig's organ) exhibited strong cRNA hybridization signals. The optic lobe of late third-instar larvae displayed ChAT immunoreactivity in Bolwig's nerve and a neuron close to the insertion site of the optic stalk. This neuron's axon ran in parallel with Bolwig's nerve to the larval optic neuropil. This neuron is likely to be a first-order interneuron of the larval visual system. Expression of the lacZ reporter gene was also detected in Bolwig's organ and the neuron stained by anti-ChAT antibody. Our observations indicate that acetylcholine may be a neurotransmitter in the larval photoreceptor cells as well as in a first-order interneuron in the larval visual system of Drosophila melanogaster.
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页码:193 / 202
页数:10
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