POLYETHYLENE-GLYCOL INDUCED FUSION OF BACTERIAL PROTOPLASTS - DIRECT SELECTION OF RECOMBINANTS

被引：22

作者：

FODOR, K

ALFOLDI, L

机构：

[1] Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, Szeged

来源：

MOLECULAR & GENERAL GENETICS | 1979年 / 168卷 / 01期

关键词：

D O I：

10.1007/BF00267933

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Direct selection for recombinants by supplemented minimal media from polyethylene-glycol (PEG)-induced fusion of protoplasts of polyauxotrophic strains of B. megaterium revealed striking physiological influences on the yield of recombinants. Cytoplasmic state of the protoplasts to be fused, rather than genetic events, determined the number of colonies obtained on the selection media. It is suggested that the physiological effects primarily influenced the ability of the fused protoplasts to revert to bacillary form. © 1979 Springer-Verlag.

引用

页码：55 / 59

页数：5

共 6 条

[1] REVERSION OF BACILLUS-MEGATERIUM PROTOPLASTS TO BACILLARY FORM [J].