SYNTHESIS, CONFORMATION AND HYDROLYTIC STABILITY OF P1,P3-DINUCLEOSIDE TRIPHOSPHATES RELATED TO MESSENGER-RNA 5'-CAP, AND COMPARATIVE KINETIC-STUDIES ON THEIR NUCLEOSIDE AND NUCLEOSIDE MONOPHOSPHATE ANALOGS

被引：64

作者：

DARZYNKIEWICZ, E

STEPINSKI, J

TAHARA, SM

STOLARSKI, R

EKIEL, I

HABER, D

NEUVONEN, K

LEHIKOINEN, P

LABADI, I

LONNBERG, H

机构：

[1] UNIV TURKU,DEPT CHEM,SF-20500 TURKU 50,FINLAND

[2] NATL RES COUNCIL CANADA,MONTREAL H4P 2R2,QUEBEC,CANADA

[3] UNIV WARSAW,DEPT CHEM,PL-02093 WARSAW,POLAND

[4] UNIV SO CALIF,SCH MED,DEPT MICROBIOL,LOS ANGELES,CA 90033

[5] ATTILA JOZSEF UNIV,DEPT INORGAN & ANALYT CHEM,H-6701 SZEGED,HUNGARY

[6] UNIV WARSAW,DEPT BIOPHYS,PL-02089 WARSAW,POLAND

来源：

NUCLEOSIDES & NUCLEOTIDES | 1990年 / 9卷 / 04期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1080/07328319008045191

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

P1. P3-Dinucleoside triphosphates, N(5 ')ppp (5’)G, have been prepared in which N is 7-Me-, 7-Et-, 7-Bn, N2, 7-diMe-or N,N,7-triMe-guanosine. Conformations of the nucleoside moieties have been determined and compared with those of the corresponding nucleoside monophosphates. The hydrolytic stability of the 7-alkylguanine ring has been studied and the origin of the structural effects elucidated by comparative kinetic studies, with monomeric nucleoside and nucleotide analogs. The mechanism of the alkaline decomposition has been established by following the cleavage of 7-methylguanosine by 1H and 13C NMR spectroscopy. © 1990, Taylor & Francis Group, LLC. All rights reserved.

引用

页码：599 / 618

页数：20

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