SEA-URCHIN (SPHAERECHINUS-GRANULARIS) CODES DIFFERENT H2B HISTONES TO ASSEMBLE SPERM AND EMBRYO CHROMATIN

被引：8

作者：

GERACI, G ^{[1
]}

LANCIERI, M ^{[1
]}

MARCHI, P ^{[1
]}

NOVIELLO, L ^{[1
]}

机构：

[1] UNIV NAPLES,IST BIOL GEN & GENET,I-80134 NAPLES,ITALY

来源：

CELL DIFFERENTIATION | 1979年 / 8卷 / 03期

关键词：

D O I：

10.1016/0045-6039(79)90045-9

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Histones from sperm and gastrula nuclei of the sea urchin Sphaerechinus granularis were analyzed by three different electrophoretic methods, stained with specific color reactions and compared with one another. The histones of the two cell types showed identical properties in none of the three analytical methods employed but the greatest differences involved the H2B type of histones. H2B from embryo chromatin had properties similar to those of the corresponding molecule from calf thymus. In the sperm chromatin no such molecule was observed but two additional ones were found that differed from embryo H2B in size, charge and specific colour reactions. The differences were such that different genes may be postulated for the synthesis of the H2B histones found in sperm and in embryo. © 1979.

引用

页码：187 / 194

页数：8

共 20 条

[1] METHOD FOR DIFFERENTIATING BETWEEN ARGININE-RICH HISTONES AND OTHERS IN POLYACRYLAMIDE-GEL [J].

BARRETT, ID ;

JOHNS, EW .

JOURNAL OF CHROMATOGRAPHY, 1973, 75 (01) :161-164

[2] ORGANIZATION OF HISTONES AND DNA IN CHROMATIN - EVIDENCE FOR AN ARGININE-RICH HISTONE KERNEL [J].

CAMERINIOTERO, RD ;

SOLLNERWEBB, B ;

FELSENFELD, G .

CELL, 1976, 8 (03) :333-347

[3] STAGE-SPECIFIC SWITCHES IN HISTONE SYNTHESIS DURING EMBRYOGENESIS OF SEA-URCHIN [J].

COHEN, LH ;

NEWROCK, KM ;

ZWEIDLER, A .

SCIENCE, 1975, 190 (4218) :994-997

[4] HIGH-RESOLUTION ELECTROPHORETIC ANALYSIS OF HISTONES FROM EMBRYOS AND SPERM OF ARBACIA-PUNCTULATA [J].

EASTON, D ;

CHALKLEY, R .

EXPERIMENTAL CELL RESEARCH, 1972, 72 (02) :502-+

[5] CHROMATIN STRUCTURE - REPEATING UNIT OF HISTONES AND DNA [J].

KORNBERG, RD .

SCIENCE, 1974, 184 (4139) :868-871

[6] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[7]

MIZUNO S, 1973, Differentiation, V1, P339, DOI 10.1111/j.1432-0436.1973.tb00130.x

[8] STAGE-SPECIFIC MESSENGER-RNAS CODING FOR SUBTYPES OF H2A AND H2B HISTONES IN SEA-URCHIN EMBRYO [J].

NEWROCK, KM ;

COHEN, LH ;

HENDRICKS, MB ;

DONNELLY, RJ ;

WEINBERG, ES .

CELL, 1978, 14 (02) :327-336

[9] HISTONES FROM SPERM OF SEA URCHIN ARBACIA LIXULA [J].

PALAU, J ;

RUIZCARR.A ;

SUBIRANA, JA .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1969, 7 (02) :209-&

[10] HIGH RESOLUTION ACRYLAMIDE GEL ELECTROPHORESIS OF HISTONES [J].

PANYIM, S ;

CHALKLEY, R .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1969, 130 (1-2) :337-&

← 1 2 →