NOREPINEPHRINE-INDUCED SYNTHESIS OF THE UNCOUPLING PROTEIN THERMOGENIN (UCP) AND ITS MITOCHONDRIAL TARGETING IN BROWN ADIPOCYTES DIFFERENTIATED IN CULTURE

被引：42

作者：

HERRON, D ^{[1
]}

REHNMARK, S ^{[1
]}

NECHAD, M ^{[1
]}

LONCAR, D ^{[1
]}

CANNON, B ^{[1
]}

NEDERGAARD, J ^{[1
]}

机构：

[1] UNIV PARIS 06,PHYSIOL COMPAREE LAB,CNRS,LA307,F-75252 PARIS,FRANCE

来源：

FEBS LETTERS | 1990年 / 268卷 / 01期

关键词：

Brown adipose tissue; Mitochondrial targeting; Nonshivering thermogenesis; Norepinephrine; Thermogenin; Uncoupling protein;

D O I：

10.1016/0014-5793(90)81031-I

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Synthesis of the brown adipocyte-specifie mitochondrial uncoupling protein thermogenin (UCP) is demonstrated here in brown adipocytes differentiated in culture from precursor cells. By immunoblotting, no UCP was detectable in untreated multilocular adipocytes. The synthesis of UCP was stimulated by norepinephrine at physiological concentrations and was observable already after 2 h. It was evident from immunoelectron microscopy that the newly synthesised protein was targeted to the mitochondrial inner membrane, demonstrating the functional competence of these cultured cells. © 1990.

引用

页码：296 / 300

页数：5

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