MODULATION OF MEMBRANE FLUIDITY BY HYDROGENATION PROCESSES .3. HYDROGENATION OF BIOMEMBRANES OF SPINACH-CHLOROPLASTS AND A STUDY OF THE EFFECT OF THIS ON PHOTOSYNTHETIC ELECTRON-TRANSPORT

A method is reported for the in situ modification of the lipids of isolated spinach chloroplast membranes. The technique is based on a direct hydrogenation of the lipid double bonds in the presence of the catalyst, chlorotris(triphenylphosphine)rhodium (I). The pattern of hydrogenation achieved suggests that the catalyst distributes amongst all of the membranes. The polyunsaturated lipids within the membranes are hydrogenated at a faster rate and at an earlier stage than are the monoenoic lipids. Whilst addition of the catalyst to the chloroplast causes an initial 10-20% decrease in Hill activity, saturation of up to 40% of the double bonds present can be accomplished without causing further significant alterations in photosynthetic electron transport processes or marked morphological changes of the chloroplast structure as observed in the electron microscope. © 1979.