HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ASSAY OF L-ALPHA-GLYCEROPHOSPHORYLCHOLINE USING A 2-STEP ENZYMATIC CONVERSION

被引：6

作者：

ABBIATI, G ^{[1
]}

FOSSATI, T ^{[1
]}

ARRIGONI, M ^{[1
]}

ROLLE, P ^{[1
]}

DOGNINI, GL ^{[1
]}

CASTIGLIONI, C ^{[1
]}

机构：

[1] SANDOZ CO,QUAL CONTROL LABS,MILAN,ITALY

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 566卷 / 02期

关键词：

D O I：

10.1016/0378-4347(91)80261-A

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A high-performance liquid chromatographic method using an enzymic reactor for determination of L-alpha-glycerophosphorylcholine in pharmaceutical forms is described. The procedure includes incubation of L-alpha-glycerophosphorylcholine with glycerophosphorylcholine phosphodiesterase (EC 3.1.4.2), giving choline and glycerophosphate, and subsequent chromatography of choline with a post-column enzymic reactor and electrochemical detection. The results obtained show a close linearity of the whole assay from 2 to 150 nmol/ml L-alpha-glycerophosphorylcholine, the sensitivity being 2 pmol per 20-mu-l of injected sample. The precision of the method in the analysis of L-alpha-glycerophosphorylcholine in pharmaceutical forms, ampoules and capsules, was 1.34 and 1.21%, respectively.

引用

页码：445 / 451

页数：7

共 8 条

[1]

ABBIATI G, 1990, BASI RAZ TER S1, V20, P1

[2]

ANSELL GB, 1982, PHOSPHOLIPIDS, P1

[3]

CANAL N, 1990, BASI RAZION TER S1, V20, P75

[4] CONCENTRATION AND ORIGIN OF CHOLINE IN RAT-BRAIN [J].