NONDESTRUCTIVE IMAGING OF INTERNAL STRUCTURES OF FROG (XENOPUS-LAEVIS) EMBRYOS BY SHADOW-PROJECTION X-RAY MICROTOMOGRAPHY

被引：2

作者：

AOKI, S

YONEDA, I

NAGAI, T

UENO, N

MURAKAMI, K

机构：

[1] UNIV TSUKUBA,INST APPL BIOCHEM,TSUKUBA,IBARAKI 305,JAPAN

[2] HOKKAIDO UNIV,FAC PHARMACEUT SCI,SAPPORO,HOKKAIDO 060,JAPAN

来源：

JAPANESE JOURNAL OF APPLIED PHYSICS PART 2-LETTERS | 1994年 / 33卷 / 4A期

关键词：

NONDESTRUCTIVE IMAGING; X-RAY MICROTOMOGRAPHY; XENOPUS-LAEVIS; EMBRYO; WHOLE-MOUNT; IMMUNOHISTOCHEMISTRY; MYOSIN; PROTEIN;

D O I：

10.1143/JJAP.33.L556

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

Nondestructive high-resolution imaging of frog (XenoPus laevis) embryos has been developed by X-ray microtomography. Shadow-projection X-ray microtomography with a brilliant fine focus laboratory X-ray source could image fine structures of Xenopus embryos which were embedded in paraffin wax. The imaging system enabled us to not only distinguish endoderm from ectoderm at the gastrula stage, but also to obtain a cross-section view of the tail bud embryo showing muscle, notochord and neural tube without staining. Furthermore, the distribution of myosin was also imaged in combination with whole-mount immunohistochemistry.

引用

页码：L556 / L558

页数：3

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