USE OF CHLOROPHYLL FLUORESCENCE TO ESTIMATE THE EFFECT OF PHOTOINHIBITION IN OUTDOOR CULTURES OF SPIRULINA-PLATENSIS

被引：62

作者：

VONSHAK, A ^{[1
]}

TORZILLO, G ^{[1
]}

TOMASELI, L ^{[1
]}

机构：

[1] UNIV FLORENCE,DISTAM,CNR,CTR STUDIO MICRORGANISMI AUTOTROFI,I-50121 FLORENCE,ITALY

来源：

JOURNAL OF APPLIED PHYCOLOGY | 1994年 / 6卷 / 01期

关键词：

FLUORESCENCE; PHOTOINHIBITION; PHOTOSYNTHESIS; SPIRULINA; TEMPERATURE;

D O I：

10.1007/BF02185901

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Chlorophyll fluorescence measurements were used to evaluate the effect of temperature on photoinhibition in Spirulina platensis cultures grown in tubular reactors outdoors. Cultures grown at 35-degrees-C during the day time showed a lower reduction in the Fv/Fm ratio as compared to cultures grown at 25-degrees-C. It is demonstrated that the lower temperature photoinhibited cells can undergo a complete recovery once transferred to low light and higher temperature. This recovery does not take place when 100 mug ml-1 chloramphenicol is added to cells. The recovery is light dependent and cells incubated in the dark at low temperature do not show a recovery in the Fv/Fm ratio. The data presented strongly support the hypothesis that photoinhibition takes place in outdoor Spirulina cultures. At the same time it is demonstrated that fluorescence measurements can be used as a fast reliable indication for photoinhibition in outdoor algal cultures.

引用

页码：31 / 34

页数：4

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